#Capto s impact resin how to#
To use the full potential of Capto S, we show how to thoroughly screen the loading parameters, conductivity and pH. Capto S is a strong cation exchanger designed for capture and intermediate purification of recombinant proteins and as a second step in MAb purification. Previously launched products on this platform include Capto Q, Capto MMC and the MabSelect™ family. The medium combines high rigidity with high dynamic binding capacity (DBC) and fast mass transfer to allow faster purification, more flexible process design and maximum cost efficiency. This has recently been used in the development of a new cation exchange medium, Capto™ S, and gives a substantial increase in binding capacity compared to the corresponding media without dextran.Ĭapto S is a chromatography medium based on the novel high flow agarose platform. A technique for significantly improving the capacity and mass transfer properties of ion exchange media is modification of the porous base matrix with a surface extender such as dextran. Diamond S and Diamond SP Mustang resins were obtained from Bestchrom (Shanghai, China). Praesto SP45 resin was obtained from Purolite (King of Prussia, PA, USA). Results show good correlation with data obtained by column. MabSelect SuRe LX resin, Capto S ImpAct resin, Tricorn 5/150 column (0.5 cm I.D.) and HiScale 26/40 column (26 cm I.D.) were obtained from Cytiva (Uppsala, Sweden). Allows fast and efficient evaluation of parameters for binding/wash/elution conditions, and resin benchmarking. Increasing expression levels of recombinant proteins and the need for improved productivity and overall process economy puts extra demand on the next generation of chromatography media for the bio-pharmaceutical industry. Supports high-throughput process development (HTPD) by allowing parallel screening of chromatographic conditions using a 96-well plate format.
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